The effect of eight different Chinese medicinal herbs (CMHs) on lymphocytes was studied in vitro using murine spleen cells. Among the studied eight CMHs, Astragalus membranaceus and Oldenlandia diffusa markedly stimulated murine spleen cells to proliferate. The responder cells for CMHs were B cells, because the response was depleted by the treatment of spleen cells with anti-immunoglobulin (Ig) antibody and complement and after purification by nylon wool column. This response was not due to contamination by lipopolysaccharide (LPS), because CMHs could stimulate C3H/HeJ spleen cells which are low responders to LPS.
CMHs enhanced the production of Ig. CMHs also enhanced the induction of allo-antigen specific cytotoxic T lymphocytes. However, CMHs had no effect on natural killer cells. Furthermore, CMHs stimulated macrophages to produce interleukin-6 and tumor necrosis factor. The electroelution of the proteins from SDS-PAGE gel showed that the active components of Oldenlandia diffusa had an apparent molecular weight of 9-200 kD and were sensitive to pronase E and NaIO4 treatment, suggesting glycoproteins in nature.
These results suggest that CMHs have immunomodulating activity in vitro and this activity could be used clinically for the modulation of immune responses.
Y. Yoshida, M.Q. Wang, J.N. Liu, B.E. Shan, U. Yamashita. Immunomodulating activity of Chinese medicinal herbs and Oldenlandia diffusa in particular. Int. J. Immunopharmacol., 19 (1997), pp. 359–370
Bai Hua She She Cao
Oldenlandia diffusa also named Hedyotis
Property: little bitter, neutral, cold, stomach meridian, large intestines meridian, small intestines meridian
Function: clear up the heat and toxic material strongly, widely used for carbuncles caused by heat, widely used for snake bite, promote urination
At high concentration, the crude preparation of the herb had inhibitory action against Ehrlich ascites carcinoma, Jitian sarcoma, and multiple kinds of leukemic cells in vitro. However, in vivo experiments showed that this herb had no significant therapeutic effects against some transplanted tumors…However, another report claimed that oldenlandia suppressed bacteriophage and inhibited ascetic liver carcinoma cells in vitro. Daily administration of the agent at the dose of 1 mg/animal for 9 days resulted in 57.4% growth inhibition of the transplanted sarcoma in mice; signification inhibition of the nuclear division of the carcinoma cells, especially that of the mitotic cells; and in more prominent cancerous degeneration and necrosis, relative to the controls.
In the book Anticancer Medicinal Herbs, some therapies are mentioned with oldenlandia and scutellaria as main ingredients for cancers of the specified areas as indicated below. The listing by cancer site should not be interpreted as meaning that the formulation is highly specific for the cancer type, only that this is what the formula had been applied for at the hospital where it was being used:
Stomach: combine oldenlandia (90 g) and imperata (60 g) or use scutellaria (30) and imperata (30)
Esophagus, rectum, and stomach: oldenlandia (70 g) and coix (30 g); plus other herbs in small quantities
Esophagus: oldenlandia (60 g), scutellaria (60 g), cycas leaf (60 g), imperata (60 g), cotton root (60 g)
Rectum: oldenlandia (60 g), scutellaria (15 g), solanum (60 g), lonicera stem (60 g), viola (15 g)
Ovary: oldenlandia (30 g), scutellaria (50 g), solanum (50 g S. nigri; 30 g S. lyrati), turtle shell (30 g)
Pleura (metastasize to): scutellaria (120 g), taraxacum (30 g)
Liver, rectum, lung: oldenlandia (60 g) and scutellaria (60 g)
Liver: oldenlandia (60 g), scutellaria (60 g), cycis (18 g), phragmites (30 g)